Laifọwọyi Kjeldahl nitrogen iṣẹ ṣiṣe ipinnu irinse:
Awọn iṣẹ ṣiṣe ti a ṣe ni apẹẹrẹ idanwo ohun elo jẹ atẹle yii: dilution, afikun reagent, distillation, titration, itusilẹ itujade, iṣiro abajade, titẹ sita.
Dilution: di digested sample in the digestive tube with distilled water.
Fi awọn reagents kun: pẹlu lye, ojutu gbigba boric acid, acid titrating.
Distillation: Ṣe ayẹwo ni tube tito nkan lẹsẹsẹ sinu nya si gbigbona lati gbe gaasi amonia jade ninu apẹẹrẹ.
Titration: Titration ti ojutu ti o gba nigba tabi lẹhin distillation.
Sisan omi: gbe omi egbin kuro lati inu tube ti ounjẹ ati ago gbigba.
Ṣe iṣiro ati tẹjade: ṣe iṣiro ati tẹjade abajade ni ibamu si iṣẹ naa.
Ilana idanwo apẹẹrẹ:
(1) Fi ohun elo sori ẹrọ ati so opo gigun ti epo.
(2) Ṣii condensate, gbe tube digestive ti o ṣofo, ṣii ohun elo akọkọ ategun afẹfẹ fun 5 ~ 10min, nu opo gigun ti epo, ki omi ṣiṣan omi jẹ iduroṣinṣin.
(3) Fi tube digestive ti o ni omi ti nmu ounjẹ ati ṣeto awọn ipele ti o baamu ati awọn iṣẹ ṣaaju ki o to bẹrẹ idanwo naa. Iṣẹ wiwa akoko gidi ti ṣiṣẹ ni akoko kanna. Ṣafikun ojutu gbigba boric acid, dilute omi ati lye sinu ohun elo Kjeldahl laifọwọyi; Amonia ti a ṣe nipasẹ distillation nya si jẹ gbigba nipasẹ isunmi pẹlu boric acid ati lẹhinna titrated pẹlu acid boṣewa kan.
(4) Awọn ṣàdánwò jẹ lori ati awọn esi ti wa ni han. O le tẹ sita, tu egbin silẹ ati nu laifọwọyi. Iboju titẹ sii paramita akọkọ ti han.
Akoko ifiweranṣẹ: Kínní-28-2022
